In vitro studies on the probiotic potential of Saccharomyces boulardii isolated from mangosteen


Abstract:

Probiotics are defined as live micro-organisms which confer a health benefit on the host. Although most probiotics are bacteria, one strain of the genus Saccharomyces, named Saccharomyces boulardii has been widely used in humans for the treatment and prevention of several gastro-intestinal disorders. But the clinical applicability and the mechanism of action of this probiotic yeast in prevention and treatment of intestinal infections have been partially elucidated. The aim of this study was to evaluate the potential of S. boulardii, isolated from mangosteen, for the prevention and treatment of selected intestinal infections. S. boulardii was isolated from the tropical fruit, mangosteen. The yeast was preliminarily identified by LPCB staining. DNA sequencing was performed for species confirmation. The conserved ITS region of the organism was amplified using PCR and sequenced by thermal cycler. The sequence was compared and identified by BLAST programme. The isolate had shown 96% sequence similarity with S. boulardii. To check the possible effectiveness of S. boulardii against diarrhoea of different etiologies, the in vitro antagonistic and antimicrobial activity against a variety of human pathogenic or opportunistic bacteria was investigated using agar spot-on lawn method and the well diffusion assay, respectively. Further, the extract prepared from S. boulardii culture filtrate was fractioned and subjected to GC-MS analysis. 15 compounds were detected, among which pyrrolopyridine methyl pyrazolone, azetidinone hexadecanoic acid and ethyl ester were found to exhibit antibacterial activity. This work was dedicated to explore the mechanism behind the use of S. boulardii as a probiotic.



Keywords:
Probiotic; Mangosteen; Gastro-intestinal disorders; S. boulardii

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How to cite this article:
Neelayadatchi C, Kanimozhi G, Sakthivel K. In vitro studies on the probiotic potential of Saccharomyces boulardii isolated from mangosteen. Journal of Biological and Information Sciences. 2012. 1 (3). 36-41.
Available from: http://archive.biolim.org/jbis/read/BOJ001A0010.