Enzymatic hydrolysate of non-edible Crotalaria mucronata seed proteins as a cost-effective nitrogenous substitute in fermentation processes


Abstract:
Peptones have been used as essential nutritive components in the fermentation media for over a century. Peptones support the microbial growth by supplying carbon, nitrogen, minerals and other growth factors. These peptones, otherwise termed as 'protein hydrolysates' are usually derived by hydrolysing the protein sources of animals, e.g. meat extract, liver hydrolysate, etc. and vegetables, e.g. soy protein, ground nuts, etc. On the contrary, this study provides an overview on the hydrolysis of non-edible seeds of Crotalaria mucronata for the production of peptone that could be utilised as functional nitrogenous source for supporting the microbial growth in the fermentation media. In this study, an immobilised proteolytic enzyme called papain was used for hydrolysis and the hydrolysate was further processed to remove the impurities and toxic contents of Crotalaria seeds. The protein hydrolysate was subsequently analysed for its efficacy in supporting the growth of micro-organisms and enzyme production.

Keywords:
Protein hydrolysate, Non-edible seeds, Crotalaria mucronata, Immobilised papain

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How to cite this article:
Selvakumar K, Anuja Devi A, Ranganayaki S, Madhan R. Enzymatic hydrolysate of non-edible Crotalaria mucronata seed proteins as a cost-effective nitrogenous substitute in fermentation processes. Journal of Biological and Information Sciences. 2014. 3 (4). 20-22.
Available from: http://archive.biolim.org/jbis/read/BOJ001A0027.