Production and analysis of microbial growth in enzyme hydrolysate of sesame oil seed cake


In today's scenario, biotech and pharmaceutical industries are facing a big challenge to produce the bioproducts as a result of presence of prions, the causative agents of bovine spongiform encephalopathy (BSE) in the culture media manufactured from animal tissue extracts. These proteinaceous, heat-resistant particles could interfere in the production of vaccines, antisera, anti-tumour agents, enzymes, organic chemicals, vitamins, etc. Therefore, it is recommended that an alternative; non-animal origin ingredients could be used for peptone production. Hence, in this current research vegetable peptone were manufactured by partial hydrolysis of selected vegetal raw materials that are rich in proteins and tested for their efficacy in bioprocesses. Besides offering physico-chemical properties adequate for an efficient hydrolysis process, the raw materials must be available worldwide on a long-term basis at a reasonable cost. And reportedly, sesame oil seed cake satisfied the above conditions; therefore, it was defatted to remove the fat content from the seeds. The oil seed flour was processed, and the protein was hydrolysed with 1% papain. The peptone obtained by enzyme hydrolysis was analysed for morphological and growth supporting properties of test organisms, such as Escherichia coli, Bacillus subtilis, Saccharomyces cerevisae and Candida albicans, and compared with commercial peptone for vegetal peptone production. A comparative study of vegetal and animal-based peptone revealed that, the growth and morphology of yeast and bacteria was at par with the conventional peptone. Thus, the vegetal peptone obtained from sesame oil seed could be used as a constituent in the dehydrated media of many fermentation processes. This could eliminate the potential risk of bovine spongiform encephalopathy.

Peptone; BSE; Fermentation media; Sesame protein

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How to cite this article:
Kavitha N, Selvakumar K, Srinivasan G, Madhan R. Production and analysis of microbial growth in enzyme hydrolysate of sesame oil seed cake. Journal of Biological and Information Sciences. 2012. 1 (1). 4-7.
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